Sloane B F, Bird J W
Am J Physiol. 1977 Apr;232(4):E423-31. doi: 10.1152/ajpendo.1977.232.4.E423.
The activities of the lysosomal acid hydrolases-cathespin D, acid phosphatase, beta-N-acetylglucosaminidase, and beta-glucuronidase-were measured in rat myometrium under the following hormonal conditions: during the estrus stage of the estrous cycle (NE); at 1,2, and 3 wk after ovariectomy; and in 3-wk postovariectomized females after hormone replacement therapy with 17 beta-estradiol (E2), progesterone (P), or E2 + P. Activities per milligram protein and per milligram DNA of the enzymes were significantly decreased after ovariectomy and were restored to the NE level or above after injecting E2 or E2 + P. Lysosomal enzyme activities did not change with hormonal state in hypophysectomized rats, suggesting that other hormones are required for mediation of enzyme activity. Acid hydrolase activities in other tissues and nonlysosomal enzyme activites in the myometrium did not fluctuate with hormonal state. Studies of lysosomal membrane integrity suggested that one population of lysosomes richer in cathepsin D and acid phosphatase and another rich in beta-N-acetylglucosaminidase and beta-glucuronidase may be present in rat myometrium. Estrogen seemed to labilize the lysosomal membrane of at least the latter of the two proposed populations of myometrial lysosomes.
在以下激素条件下，测定了大鼠子宫肌层中溶酶体酸性水解酶（组织蛋白酶D、酸性磷酸酶、β-N-乙酰氨基葡萄糖苷酶和β-葡萄糖醛酸酶）的活性：发情周期的发情期（NE）；卵巢切除术后1周、2周和3周；以及在卵巢切除术后3周的雌性大鼠中，用17β-雌二醇（E2）、孕酮（P）或E2 + P进行激素替代治疗后。卵巢切除术后，每毫克蛋白质和每毫克DNA的酶活性显著降低，注射E2或E2 + P后恢复到NE水平或更高。垂体切除的大鼠中，溶酶体酶活性不随激素状态变化，这表明酶活性的调节需要其他激素。其他组织中的酸性水解酶活性和子宫肌层中的非溶酶体酶活性不随激素状态波动。溶酶体膜完整性研究表明，大鼠子宫肌层中可能存在一类富含组织蛋白酶D和酸性磷酸酶的溶酶体，以及另一类富含β-N-乙酰氨基葡萄糖苷酶和β-葡萄糖醛酸酶的溶酶体。雌激素似乎使子宫肌层溶酶体中至少后一类溶酶体的膜不稳定。
