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["通过等电聚焦对‘tricholysine’进行分级分离"]

[Fractionation of "tricholysine" by isoelectric focusing].

作者信息

Stepanova Ts N, Iulikova E P, Maksimova R A, Silaeva A B, Andreenko G V

出版信息

Biokhimiia. 1977 Jan;42(1):167-70.

PMID:557999
Abstract

A possibility to fractionate fibrinolytically and thrombolytically active complex "tricholysin" into five components, differing in iso-points and in enzymatic activity, is demonstrated by means of isoelectric focusing using ampholine solution within pH range 3.0-10.0. Homogenous fraction IV (isopoint 6.8-7.0) has a low caseinolytic activity and a high specific fibrinolytic and esterase activities. This fraction is characterized with a high ability of plasminogen activation. Serine, threonine, alanine and valine are found to prevail in amino acid composition of the fraction IV, its molecular weight being 39000.

摘要

通过在pH值3.0 - 10.0范围内使用两性电解质溶液进行等电聚焦,证明了具有纤维蛋白溶解和血栓溶解活性的复合物“tricholysin”有可能被分离成五个在等电点和酶活性方面不同的组分。均一的组分IV(等电点6.8 - 7.0)具有低酪蛋白溶解活性以及高特异性纤维蛋白溶解和酯酶活性。该组分的特征在于具有高纤溶酶原激活能力。发现组分IV的氨基酸组成中丝氨酸、苏氨酸、丙氨酸和缬氨酸占主导,其分子量为39000。

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