Takeuchi J, Sobue M, Shamoto M, Yoshida M, Sato E, Leighton J
Cancer Res. 1977 May;37(5):1507-12.
Morphological observations and biochemical analysis were made on glycosaminoglycans produced by MDCK cells of dog kidney origin growing on a glass surface as a mosaic of epithelium with many multicellular hemishperical vesicles. MDCK cells synthesized glycosaminoglycans, which consisted mainly of heparan sulfate and hyaluronic acid. The majority of the substances were contained in a cell-surface component removable with ethylenediaminetetraacetic acid-trypsin. In the radioautograph of tissue sections, high radioactivity of 35SO4 was observed on the medium-bathed cell surface, where Alcian blue-strained material could be observed. Ultrastructurally, the surface of microvillous processes which were abundant on the cell surface in contact with the medium was stained with ruthenium red. A small amount of chondroitin 4- and 6-sulfates were also synthesized. After 24 hr, the majority of chondrotin [35S] sulfates newly formed were secreted into the cultured medium, whereas haparan [35S] sulfate was released much less, remaining as a cellular component. The biological roles of glyconsaminoglycans produced by epithelial cells are discussed.
对源自犬肾的MDCK细胞在玻璃表面生长形成的上皮镶嵌结构及许多多细胞半球形囊泡产生的糖胺聚糖进行了形态学观察和生化分析。MDCK细胞合成了糖胺聚糖，其主要由硫酸乙酰肝素和透明质酸组成。大部分物质包含在可被乙二胺四乙酸 - 胰蛋白酶去除的细胞表面成分中。在组织切片的放射自显影中，在与培养基接触的细胞表面观察到高放射性的35SO4，同时也能观察到阿尔辛蓝染色的物质。超微结构上，与培养基接触的细胞表面丰富的微绒毛突起表面被钌红染色。也合成了少量的硫酸软骨素4 - 硫酸酯和6 - 硫酸酯。24小时后，新形成的大部分硫酸软骨素[35S]硫酸酯分泌到培养基中，而硫酸乙酰肝素[35S]硫酸酯释放较少，仍作为细胞成分保留。讨论了上皮细胞产生的糖胺聚糖的生物学作用。
