通过间接固相放射免疫测定法检测感染Semliki森林病毒的细胞中的双链RNA：一种干扰素检测方法。
Detection of double-stranded RNA in Semliki Forest virus-infected cells by an indirect solid phase radioimmunoassay: an assay for interferon.
作者信息
Williams B R, Kalmakoff J
出版信息
Intervirology. 1977;8(2):110-6. doi: 10.1159/000148885.
PMID:558176
Abstract
An indirect solid phase radioimmunoassay using rabbit antibodies to the synthetic double-stranded RNA poly rI-rC and 125I-labelled sheep anti-rabbit IgG could detect 1 ng of poly rI-rC. This assay could also detect the presence of double-stranded RNA in Semliki Forest virus-infected chick fibroblasts and can be used as an assay for interferon activity.
摘要
一种间接固相放射免疫测定法，使用针对合成双链RNA多聚rI-rC的兔抗体和125I标记的羊抗兔IgG，能够检测到1纳克的多聚rI-rC。该测定法还能检测到感染Semliki森林病毒的鸡成纤维细胞中双链RNA的存在，可作为一种干扰素活性测定法。
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