利用塞姆利基森林病毒核糖核酸合成抑制优化干扰素测定法。
Optimization of the interferon assay using inhibition of Semliki forest virus-ribonucleic acid synthesis.
作者信息
Koblet H, Kohler U, Wyler R
出版信息
Appl Microbiol. 1972 Sep;24(3):323-7. doi: 10.1128/am.24.3.323-327.1972.
Abstract
The method for assaying chicken interferon by its inhibition of viral ribonucleic acid (RNA) synthesis was optimized for the chicken embryo fibroblast-Semliki Forest virus (SFV) system, with respect to time, multiplicity of infection, and addition of actinomycin D and (3)H-uridine. Incorporation of (3)H into viral RNA is reproducible, and amounts to 10(4) to 2 x 10(4) counts per min per uninhibited culture per 1 muCi per 6 hr. The assay may be carried out in less than 1 day and is sensitive (0.05 units/ml) and exact (+/-20% of the mean titers on different days); it can be used for purification procedures as well.
摘要
通过抑制病毒核糖核酸（RNA）合成来检测鸡干扰素的方法，针对鸡胚成纤维细胞 - 辛德毕斯病毒（SFV）系统，在时间、感染复数以及放线菌素D和（3）H - 尿苷的添加方面进行了优化。（3）H掺入病毒RNA的过程具有可重复性，每6小时每1微居里每未受抑制的培养物每分钟可达10⁴至2×10⁴计数。该检测可在不到1天的时间内完成，灵敏度高（0.05单位/毫升）且准确（不同日期平均滴度的±20%）；它也可用于纯化程序。
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Optimization of the interferon assay using inhibition of Semliki forest virus-ribonucleic acid synthesis.利用塞姆利基森林病毒核糖核酸合成抑制优化干扰素测定法。
Appl Microbiol. 1972 Sep;24(3):323-7. doi: 10.1128/am.24.3.323-327.1972.
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本文引用的文献
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QUANTITATIVE HAEMADSORPTION, A NEW ASSAY TECHNIQUE. I. ASSAY OF INTERFERON.定量血细胞吸附法，一种新的检测技术。I. 干扰素检测
Virology. 1964 Dec;24:589-97. doi: 10.1016/0042-6822(64)90212-0.
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Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon.痘苗病毒蚀斑形成及其受干扰素抑制的研究。III. 一种简化的干扰素蚀斑抑制试验。
Virology. 1963 Mar;19:302-9. doi: 10.1016/0042-6822(63)90068-0.
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