Thi Son N, Behrens U
Nahrung. 1977;21(1):69-78.
The well known fodder yeasts do not accumulate extracellular amylolytic enzymes and are therefore unable to utilize starches for the production of microbial biomasses. Some of the Endomycopsis yeasts produce glucoamylase but their specific growth rate is so small that continuous cultivation in starch containing media results in low productivites. In the "Symba-process" this drawback is overcome by a twostage fermentation process. In the first stage only Endomycopsis fibuliger yeasts are continuously cultivated and in the second stage the main part of the starch containing medium is converted to microbial biomasses by a mixed culture consisting of fodder yeasts (p.a. Candida utilis) and Endomycopsis fibuliger transferred continously from the first stage. In this work methods for evaluating conditions of cultivation of such a process are presented and a two stage continuous fermentation of a starch containing medium is performed. In the second stage with a dilution rate of D Y 0,174 h-1 a productivity of 1,7 g dry biomasses/1-h was attained Methods for improving the productivity are discussed.
众所周知,饲料酵母不会积累细胞外淀粉酶,因此无法利用淀粉来生产微生物生物质。一些拟内孢霉酵母能产生葡糖淀粉酶,但其比生长速率非常低,以至于在含淀粉培养基中进行连续培养时产量很低。在“辛巴工艺”中,通过两阶段发酵工艺克服了这一缺点。在第一阶段,仅对产黄丝孢酵母进行连续培养,在第二阶段,由饲料酵母(产朊假丝酵母)和从第一阶段连续转移过来的产黄丝孢酵母组成的混合培养物将含淀粉培养基的主要部分转化为微生物生物质。在这项工作中,介绍了评估此类工艺培养条件的方法,并对含淀粉培养基进行了两阶段连续发酵。在第二阶段,稀释率为D Y 0.174 h-1时,生物质干重产量达到1.7 g/(1·h),并讨论了提高产量的方法。