Teale J D, Clough J M, Marks V
Br J Clin Pharmacol. 1977 Apr;4(2):169-72. doi: 10.1111/j.1365-2125.1977.tb00690.x.
1 The cytotoxic agent, vinblastine, was conjugated to albumin, using the Mannich reaction. Rabbits immunized with two conjugates, containing differing amounts of hapten, produce antibodies which bound [3H]-vinblastine. 2 Antisera from one rabbit cross-reacted with both vinblastine and vincristine and were used to develop radioimmunoassays for measuring their concentration in plasma. 3 The antisera showed no cross-reactivity with other alkaloids or cytotoxic drugs and provided assays sensitive to a concentration of 2.1 ng vinblastine or 3.8 ng vincristine/ml of plasma added direct to the assay tubes. 4 This is sufficiently sensitive to permit the measurement of plasma vinblastine levels for up to 24 h after the intravenous administration of 15 mg of the drug.
利用曼尼希反应将细胞毒性药物长春碱与白蛋白偶联。用含有不同量半抗原的两种偶联物免疫兔子，产生了能结合[3H] - 长春碱的抗体。
一只兔子的抗血清与长春碱和长春新碱均发生交叉反应，并用于开发放射免疫分析法以测定它们在血浆中的浓度。
该抗血清与其他生物碱或细胞毒性药物无交叉反应，对直接加入测定管中的血浆中浓度为2.1 ng长春碱或3.8 ng长春新碱/ml具有灵敏性。
这一灵敏度足以在静脉注射15 mg该药物后长达24小时内测定血浆长春碱水平。
