血浆和尿液中博来霉素的放射免疫测定
Radioimmunoassay of bleomycin in plasma and urine.
作者信息
Teale J D, Clough J M, Marks V
出版信息
Br J Cancer. 1977 Jun;35(6):822-7. doi: 10.1038/bjc.1977.124.
Antibodies to bleomycin were raised by immunization of sheep and rabbits with bleomycin-albumin conjugates. The combination of a high-titre, high-avidity sheep antiserum and iodinated bleomycin produced a radioimmunoassay sensitive to 8 ng of bleomycin per ml of plasma or urine. Untreated specimens (100 microliter) of plasma or urine could be added directly to the assay tubes. The antiseerum was specific for bleomycin and showed no cross-reaction with other anticancer agents used in combination chemotherapy. Over a concentration range of 20-100 ng/ml, recovery of bleomycin from plasma was 110% and from urine, 93%. Repeated assay of plasma samples showed a decrease in bleomycin levels unless the samples were kept at 4 degrees C or below. Assay of bleomycin levels in plasma and urine from patients under treatment with bleomycin showed similarities with results reported using a microbiological assay. The radioimmunoassay offers a more reliable, rapid and sensitive method for the measurement of bleomycin.
通过用博来霉素 - 白蛋白偶联物免疫绵羊和兔子制备抗博来霉素抗体。高滴度、高亲和力的绵羊抗血清与碘化博来霉素相结合，产生了一种对每毫升血浆或尿液中8纳克博来霉素敏感的放射免疫测定法。血浆或尿液的未处理标本（100微升）可直接加入测定管中。抗血清对博来霉素具有特异性，与联合化疗中使用的其他抗癌药物无交叉反应。在20 - 100纳克/毫升的浓度范围内，血浆中博来霉素的回收率为110%，尿液中为93%。除非样本保存在4℃或更低温度，否则对血浆样本进行重复测定显示博来霉素水平会下降。对接受博来霉素治疗的患者的血浆和尿液中的博来霉素水平进行测定，结果与使用微生物测定法报告的结果相似。放射免疫测定法为博来霉素的测量提供了一种更可靠、快速且灵敏的方法。
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