Roth-Schechter B F, Mandel P
Experientia. 1977 May 15;33(5):627-9. doi: 10.1007/BF01946540.
The effect of pentobarbital was studied in a mixed population of nerve and glial cells dissociated from brains of 7-day chick embryos and maintained in culture. Pentobarbital-Na was added in various concentrations ranging from 5 X 10(-5) M to 1 X 10(-3) M. The neuronal density was monitored by counting the neurons, neuronal identity was established by staining for Nissl Bodies and acetylcholinesterase. Over a culture period of 3 weeks, it was found that the barbiturate exerts a preferential dose-dependent cytotoxic effect on neurons.
在从7日龄鸡胚大脑中分离并培养的神经细胞和神经胶质细胞混合群体中研究了戊巴比妥的作用。添加了浓度范围从5×10⁻⁵ M至1×10⁻³ M的戊巴比妥钠。通过计数神经元来监测神经元密度，通过对尼氏体和乙酰胆碱酯酶进行染色来确定神经元身份。在3周的培养期内，发现巴比妥酸盐对神经元具有优先的剂量依赖性细胞毒性作用。
