Mareel M, de BRUYNE G, de Ridder L
Oncology. 1977;34(1):6-9. doi: 10.1159/000225171.
We have attempted to quantitate malignant invasion in vitro. Therefore we adapted the 51Cr-release cytotoxicity test to organotypical culture systems, that proved useful for morphological analysis of invasion. We counted 51Cr-release from labeled fragments of embryonic chick heart and mesonephros, cultured either on semi-solid or in fluid medium, during their invasion by HeLa cells. Although demonstrated morphologically, malignant invasion did not bring about significant changes of 51Cr-release as compared to control cultures without HeLa cells. The failure of the 51Cr-release test in our experimental device might shed some light on the mechanisms of malignant invasion.
我们尝试对体外恶性侵袭进行定量分析。因此，我们将⁵¹Cr释放细胞毒性试验应用于器官型培养系统，该系统被证明对侵袭的形态学分析很有用。我们在HeLa细胞侵袭培养于半固体或液体培养基中的胚胎鸡心脏和中肾的标记片段过程中，对⁵¹Cr的释放进行了计数。尽管从形态学上得到了证实，但与没有HeLa细胞的对照培养相比，恶性侵袭并未导致⁵¹Cr释放的显著变化。在我们的实验装置中⁵¹Cr释放试验的失败可能有助于揭示恶性侵袭的机制。
