Rosenbaum R M, Picciano P, Kress Y, Wittner M
Anat Rec. 1977 Jun;188(2):241-61. doi: 10.1002/ar.1091880209.
Fragments of adult rabbit lung, composed chiefly of terminal airway obtained by a trypsin digestion technique were maintained on collagen-coated cellulose sponges in Ham's F12 medium. Cell-sponge associations were examined with light microscopy, scanning and transmission electron microscopy over a period from 6 to 28 days. After an initial 24- to 48-hour period of cell migration from the airway fragment, sponge matrices became lined with cells suggestive of alveolar macrophages. After one week in culture, cysts appeared to be composed entirely of type 2 epithelial cells. These were characterized by a microvillous apical border and an elaborate junctional complex. The lumen of these cysts contained both myelin-like lamellar configurations and tubular myelin structures such as have been described from pulmonary washings. Consistent with the age of the sponge cultures, one or more cyst types described as young, middle and late could be found simultaneously. Middle aged cysts showed signs of active secretion into the lumen. Late cysts showed changes in the epithelium comprising the cyst wall suggestive of a cell type intermediate between type 1 and type 2 epithelial cells.
通过胰蛋白酶消化技术获取的成年兔肺组织碎片，主要由终末气道构成，将其置于涂有胶原蛋白的纤维素海绵上，培养于哈姆氏F12培养基中。在6至28天的时间段内，利用光学显微镜、扫描电子显微镜和透射电子显微镜对细胞 - 海绵联合体进行检查。在最初的24至48小时内，细胞从气道碎片迁移，之后海绵基质表面排列着类似肺泡巨噬细胞的细胞。培养一周后，囊肿似乎完全由Ⅱ型上皮细胞组成。这些细胞的特征是具有微绒毛状的顶端边界和精细的连接复合体。这些囊肿的腔内既有髓鞘样板层结构，也有管状髓鞘结构，就像在肺灌洗中所描述的那样。与海绵培养的时间一致，可以同时发现一种或多种被描述为年轻、中年和晚期的囊肿类型。中年囊肿显示出向腔内活跃分泌的迹象。晚期囊肿显示构成囊肿壁的上皮细胞发生变化，提示为介于Ⅰ型和Ⅱ型上皮细胞之间的一种细胞类型。
