Rose G G, Yajima T
In Vitro. 1978 Jul;14(7):557-80. doi: 10.1007/BF02617916.
Long-term (more than 75 days) recirculating circumfusion system cultures of 15-day fetal mouse lungs gave rise to terminal bronchiolar-alveolar (TB-A) units detached from the respiratory tree. TB-A units established in serum-free microenvironments under reconstructed cellophane membranes became especially flat and optically accessible in fast-flowing 6-chamber systems devoid of a pulse pressure. Time-lapse cinephase movies showed that the alveoli was maintained debris-free by the phagocytosis of alveolar macrophages which entered the alveoli by penetrating the pneumocytic walls. The terminal bronchioles with active ciliary cells communicated with the alveoli and together they formed a flat fluid-filled tubule-like closed system composed of ultrastructurally typical differentiated cellular constituents, viz. types I and II pneumocytes, endocrine-like cells, ciliary cells. Clara cells, and septal cells. TB-A units were observed throughout 150 days of cultivation at which time their phase morphological differentiation remained stable and their functional activity observable as smooth-muscle contractions, ciliation, and alveolar macrophage phagocytosis. A 1000-foot motion-picture film complementing the descriptions of the TB-A units and the alveolar macrophages in the text is deposited with the Motion Picture Film Rental Collection of the Tissue Culture Association, Inc., at the W. Alton Jones Cell Science Center, Lake Placid, New York.
对15日龄胎鼠肺进行长期（超过75天）循环灌注系统培养，可产生与呼吸树分离的终末细支气管-肺泡（TB-A）单位。在重建的玻璃纸膜下无血清微环境中建立的TB-A单位，在无脉压的快速流动6室系统中变得特别扁平且便于光学观察。延时电影显示，肺泡巨噬细胞穿透肺细胞壁进入肺泡，通过吞噬作用使肺泡保持无碎片状态。具有活跃纤毛细胞的终末细支气管与肺泡相通，它们共同形成一个扁平的充满液体的管状封闭系统，由超微结构典型的分化细胞成分组成，即I型和II型肺细胞、类内分泌细胞、纤毛细胞、克拉拉细胞和间隔细胞。在整个150天的培养过程中都观察到了TB-A单位，此时它们的相形态分化保持稳定，其功能活性表现为平滑肌收缩、纤毛运动和肺泡巨噬细胞吞噬作用。一部1000英尺的电影胶片补充了文中对TB-A单位和肺泡巨噬细胞的描述，该胶片存放在纽约普拉西德湖W.奥尔顿·琼斯细胞科学中心的组织培养协会电影胶片租赁收藏库中。
