宿主诱导的新城疫病毒病毒粒子多肽修饰。

Host induced modifications of Newcastle disease virus virion polypeptides.

作者信息

Terry G M

出版信息

Arch Virol. 1977;53(4):295-303. doi: 10.1007/BF01315628.

Abstract

The polypeptide composition of Newcastle Disease Virus (NDV) virions grown in two host cell cystems--chorioallantlic membrane (CAM) and BHK-21 cells--was studied. Two strains of virus were compared, one highly virulent, the other completely avirulent. No significant differences in the polypeptide composition of the two strains of virus could be detected. However, differences were found in virions grown in different hosts, the same differences being found in both strains. An additional polypeptide is found in BHK grown virus which is not present in CAM grown virus and this is associated with a decreased relative amount of nucleocapsid protein in BHK grown virus. The possibility of this new polypeptide being a degradation product of the nucleocapsid protein is discussed. BHK grown virions also contain increased amounts of a polypeptide migrating to a position which might be expected of the FO precursor glycoprotein. However, in contrast to the FO polypeptide, this polypeptide does not appear to be glycosylated.

摘要

对在两种宿主细胞系统——绒毛尿囊膜（CAM）和BHK - 21细胞中培养的新城疫病毒（NDV）病毒粒子的多肽组成进行了研究。比较了两株病毒，一株高毒力，另一株完全无毒力。未检测到这两株病毒在多肽组成上有显著差异。然而，在不同宿主中培养的病毒粒子存在差异，两株病毒都有相同的差异。在BHK细胞中培养的病毒中发现了一种在CAM细胞中培养的病毒中不存在的额外多肽，这与BHK细胞中培养的病毒中核衣壳蛋白相对含量的减少有关。讨论了这种新多肽是核衣壳蛋白降解产物的可能性。BHK细胞中培养的病毒粒子还含有增加量的一种迁移到可能是F0前体糖蛋白预期位置的多肽。然而，与F0多肽不同，这种多肽似乎没有糖基化。