Cohen E, Sverdlov E, Wool D
Biochem Genet. 1977 Apr;15(3-4):253-64. doi: 10.1007/BF00484457.
Two electrophoretically fast-migrating, nonspecific esterases were detected in two strains of the flour beetle Tribolium castaneum and designated F (fast) and S (slow) according to their relative migration distances. Both isozymes are associated with the alimentary canal and display ontogenetic changes. Their activity is very low in the egg stage, increases in the larva, and declines dramatically in the pharate pupa and pupa. The overall activity in the pupal stage is low, yet increases gradually throughout this period. In the adult, the activity of the esterases rises sharply. The larval and adult F and S isozymes were found to hydrolyze alpha- and beta-naphthylacetate and alpha-naphthylpropionate with almost equal capacity. alpha-Naphthyl laurate was cleaved by the F enzyme of both larvae and adults. The F and S were insensitive to inhibitors of arylesterases and cholinesterases and were markedly inhibited by the organophosphate di-isopropylphosphorofluoridate (DFP) and could be classified as carboxylesterases. Differential sensitivities of larval and adult esterases to urea and heat treatment as well as to DFP may indicate the expression of different genes during metamorphosis.
在两种赤拟谷盗（Tribolium castaneum）品系中检测到两种电泳迁移速度快的非特异性酯酶，根据它们相对的迁移距离分别命名为F（快）和S（慢）。这两种同工酶都与消化道相关，并呈现个体发育变化。它们在卵期活性很低，在幼虫期增加，在近蛹期和蛹期急剧下降。蛹期的总体活性较低，但在此期间逐渐增加。在成虫中，酯酶的活性急剧上升。已发现幼虫和成虫的F和S同工酶对α-和β-萘乙酸酯以及α-萘丙酸酯的水解能力几乎相同。月桂酸α-萘酯可被幼虫和成虫的F酶裂解。F和S对芳基酯酶和胆碱酯酶的抑制剂不敏感，但被有机磷酸酯二异丙基氟磷酸酯（DFP）显著抑制，可归类为羧酸酯酶。幼虫和成虫酯酶对尿素、热处理以及DFP的不同敏感性可能表明在变态过程中不同基因的表达。
