β-内酰胺抗生素的无细胞生物合成研究。
Studies on the cell-free biosynthesis of beta-lactam antibiotics.
作者信息
Bost P E, Demain A L
出版信息
Biochem J. 1977 Mar 15;162(3):681-7. doi: 10.1042/bj1620681.
Abstract
Cell walls of Cephalosporium acremonium mycelia were lysed by enzyme preparations from either Helix pomatia (snail) digestive juice or Cytophaga. The yield of protoplasts depended on the lytic-enzyme preparation and the age of the culture, and it increased after the mycelia were pretreated with dithiothreitol. A cell-free preparation, obtained by osmotic lysis of protoplasts, synthesized labelled penicillin N from L-[14C]valine. Approx. 0.03-0.06% of the amino acid was incorporated into penicillin N. Under conditions of penicillin N synthesis, the broken-protoplast preparation failed to produce significant amounts of cephalosporin C or its precursors, deacetylcephalosporin C and deacetoxycephalosporin C.
摘要
顶头孢霉菌丝体的细胞壁可被来自苹果螺（蜗牛）消化液或噬纤维菌的酶制剂裂解。原生质体的产量取决于裂解酶制剂和培养物的菌龄，并且在用二硫苏糖醇预处理菌丝体后产量会增加。通过原生质体的渗透裂解获得的无细胞制剂从L-[14C]缬氨酸合成了标记的青霉素N。约0.03 - 0.06%的氨基酸被掺入青霉素N中。在青霉素N合成的条件下，破碎的原生质体制剂未能产生大量的头孢菌素C或其前体，去乙酰头孢菌素C和去乙酰氧基头孢菌素C。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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2
Synthesis of D-delta-amino-delta-carboxyvalerylglycine (a degradation product of cephalosporin N) and of DL-delta-amino-delta-carboxyvaleramide.D-δ-氨基-δ-羧基戊酰甘氨酸（头孢菌素N的一种降解产物）和DL-δ-氨基-δ-羧基戊酰胺的合成。
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Behaviour of alpha-aminoadipylcysteine and glutamylcysteine in the presence of intact and disrupted mycelium of a Cephalosporium sp.α-氨基己二酰半胱氨酸和谷氨酰半胱氨酸在完整及破碎的头孢霉属真菌菌丝体存在情况下的行为
Biochem J. 1969 May;112(4):389-96. doi: 10.1042/bj1120389.
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