顶头孢霉中无细胞体系将δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸转化为具有异青霉素N特性的抗生素。
Cell-free conversion of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium.
作者信息
Konomi T, Herchen S, Baldwin J E, Yoshida M, Hunt N A, Demain A L
出版信息
Biochem J. 1979 Nov 15;184(2):427-30. doi: 10.1042/bj1840427.
Abstract
Cell-free extracts of antibiotic-negative mutants of Cephalosporium acremonium converted delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (LLD-tripeptide) into an antibiotic that was destroyed by penicillinase. The enzymic activity of the extracts was destroyed by boiling, but was not inhibited by cycloheximide. LLL-Tripeptide was totally inactive as substrate. The product resembled isopenicillin N, but not penicillin N, in its antibacterial spectrum. We propose that isopenicillin N is the first product of cyclization of LLD-tripeptide.
摘要
顶头孢霉抗生素阴性突变体的无细胞提取物将δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸（LLD-三肽）转化为一种能被青霉素酶破坏的抗生素。提取物的酶活性经煮沸后被破坏，但不受放线菌酮抑制。LLL-三肽作为底物完全无活性。该产物在抗菌谱上类似于异青霉素N，而非青霉素N。我们提出异青霉素N是LLD-三肽环化的首个产物。
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Cell-free conversion of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine into an antibiotic with the properties of isopenicillin N in Cephalosporium acremonium.顶头孢霉中无细胞体系将δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸转化为具有异青霉素N特性的抗生素。
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2
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本文引用的文献
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Aminoadipylpenicillin in penicillin fermentations.青霉素发酵中的氨基己二酰青霉素。
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Synthesis of delta-(alpha-aminoadipyl)cysteinylvaline and its role in penicillin biosynthesis.δ-（α-氨基己二酰）半胱氨酰缬氨酸的合成及其在青霉素生物合成中的作用。
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6
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Studies on the cell-free biosynthesis of beta-lactam antibiotics.β-内酰胺抗生素的无细胞生物合成研究。
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Cell-free ring expansion of penicillin N to deacetoxycephalosporin C by Cephalosporium acremonium CW-19 and its mutants.顶头孢霉CW-19及其突变体将青霉素N无细胞环扩展为去乙酰氧基头孢菌素C
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Synthesis of tritium-labelled isopenicillin N, penicillin N and 6-aminopenicillanic acid.氚标记的异青霉素N、青霉素N和6-氨基青霉烷酸的合成。
Biochem J. 1975 Dec;151(3):729-39. doi: 10.1042/bj1510729.
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