Luiken G A, McMillan R, Lightsey A L, Gordon P, Zevely S, Schulman I, Gribble T J, Longmire R L
Blood. 1977 Aug;50(2):317-25.
A method for the measurement of immunoglobulin G associated with gel-filtered platelets is described and finding in 70 control subjects and 37 patients with immune thrombocytopenic purpura (ITP) are reported. Control platelet-associated IgG (PAIgG) levels (nanograms IgG per 10(9) platelets) averaged (+/-SD) 1231+/-424; samples studied after 24 and 48 hr remained within the control range. PAIgG values of 19 adult and 12 childhood patients with chronic ITP averaged 4711+/-3025 and 4923+/-3955, respectively, and differed significantly from controls (p less than 0.001). There was an inverse correlation between PAIgG values and the chronic ITP patient's platelet count. Six patients with childhood acute ITP had PAIgG levels ranging from 5588 to 56,250 and appeared to represent a different statistical population from those with chronic ITP. In chronic ITP patients responding to splenectomy, there was an immediate normalization of PAIgG levels; however, a certain percentage of patients studied several months after splenectomy evidenced elevated PAIgG levels in association with normal platelet counts. These data showed that the direct measurement of platelet associated antibody is a useful technique in the diagnosis and follow-up of patients with chronic ITP. Preliminary studies in patients with acute ITP have suggested that this method may be useful in differentiating acute and chronic childhood ITP.
本文描述了一种测量与凝胶过滤血小板相关的免疫球蛋白G的方法，并报告了在70名对照受试者和37名免疫性血小板减少性紫癜（ITP）患者中的研究结果。对照者的血小板相关IgG（PAIgG）水平（每10⁹个血小板中IgG的纳克数）平均为（±标准差）1231±424；在24小时和48小时后研究的样本仍在对照范围内。19名成年慢性ITP患者和12名儿童慢性ITP患者的PAIgG值分别平均为4711±3025和4923±3955，与对照组有显著差异（p＜0.001）。PAIgG值与慢性ITP患者的血小板计数呈负相关。6名儿童急性ITP患者的PAIgG水平在5588至56250之间，似乎代表了与慢性ITP患者不同的统计学群体。在接受脾切除术有反应的慢性ITP患者中，PAIgG水平立即恢复正常；然而，在脾切除术后几个月研究的一定比例的患者显示，PAIgG水平升高且血小板计数正常。这些数据表明，直接测量血小板相关抗体是慢性ITP患者诊断和随访中的一项有用技术。对急性ITP患者的初步研究表明，该方法可能有助于区分儿童急性和慢性ITP。
