Long J C, Hall C L, Brown C A, Stamatos C, Weitzman S A, Carey K
N Engl J Med. 1977 Aug 11;297(6):295-9. doi: 10.1056/NEJM197708112970602.
We examined 90 serums from patients with Hodgkin's disease for immune complexes and for reactivity with established monolayer tissue cultures prepared from the tumor. All 23 serums with immune complex levels greater than 20 microgram per milliliter were found to react with cultured cells of patients with Hodgkin's disease when tested with antiserums against immunoglobulin heavy and light chains and the C3 component of complement. Five of 11 serums with borderline elevations of immune complexes (10 to 20 microgram per milliliter) and only four of 56 with levels less than 10 microgram per milliliter reacted. Absorption of patients' serums with cultured cells removed immune complexes and eliminated binding to monolayers. Immune-complex-containing serums from 19 control patients did react with cultured cells of patients with Hodgkin's disease; none of serums reacted with normal cultured spleen. Antibodies within complement-containing immune complexes in serums of patients with Hodgkin's disease react with an antigen on the surface of cultured cells of such patients.
我们检测了90份霍奇金病患者的血清，以寻找免疫复合物，并检测其与从肿瘤制备的已建立的单层组织培养物的反应性。当用抗免疫球蛋白重链和轻链以及补体C3成分的抗血清进行检测时，发现所有免疫复合物水平高于每毫升20微克的23份血清都能与霍奇金病患者的培养细胞发生反应。11份免疫复合物水平处于临界升高状态（每毫升10至20微克）的血清中有5份发生反应，而56份水平低于每毫升10微克的血清中只有4份发生反应。用培养细胞吸收患者血清可去除免疫复合物并消除与单层细胞的结合。19名对照患者的含免疫复合物血清确实能与霍奇金病患者的培养细胞发生反应；没有一份血清能与正常培养的脾脏发生反应。霍奇金病患者血清中含补体的免疫复合物中的抗体与这类患者培养细胞表面的一种抗原发生反应。
