Kindahl H, Granström E, Edqvist L E, Gustafsson B, Aström G, Stabenfeldt G
Acta Vet Scand. 1977;18(2):274-86. doi: 10.1186/BF03548456.
The effect of intrauterine iodine infusion on estrous cycle length was studied in four cows. The infusions were performed at various times of the estrous cycle: early, middle, late, and during luteolysis. Blood samples were drawn every third hour from the jugular vein. Progesterone and 15-keto-13,14-dihydroprostaglandin F (the main metabolite of PGF) were measured to monitor luteal activity and prostaglandin release. No release of prostaglandins was observed immediately following intrauterine infusion. Infusion in two cows on day 5 of the estrous cycle resulted in prostaglandin release after 54 and 69 hrs., respectively, followed by luteal regression and the occurrence of estrus at approx. five days after infusion. Infusions performed on days 11 or 12 resulted in prostaglandin release after 147 and 120 hrs., respectively, followed by luteolysis and heat after a 19 day estrous cycle. Infusion in two cows at days 16 and 17 resulted in prostaglandin release after 117 hrs. in both animals. One cycle was prolonged whereas the other cycle was normal in duration. One cow infused on day 20 following the occurrence of the first prostaglandin surge had a cycle length of 26 days, whereas another cow infused on day 20 was not affected because luteolysis was essentially complete by the time of infusion. One animal infused on day 5 did not respond to the iodine infusion. In this animal, however, the corpus luteum was not completely developed prior to the infusion. From this study it can be concluded: 1) intrauterine iodine infusions performed after the development of a progesterone secreting corpus luteum result in prostaglandin release within three to six days with the subsequent occurrence of luteolysis; 2) luteolysis was in all cases observed in connection with prostaglandin F release of the same order of magnitude and duration as during normal luteolysis. kw|Keywords|k]prostaglandin release; k]progesterone; k]cow; k]es trous cycle; k]iodine infusion
在四头奶牛身上研究了宫内输注碘对发情周期长度的影响。输注在发情周期的不同时间进行：早期、中期、晚期以及黄体溶解期。每隔三小时从颈静脉采集血样。测量孕酮和15-酮-13,14-二氢前列腺素F（PGF的主要代谢产物）以监测黄体活性和前列腺素释放。宫内输注后未立即观察到前列腺素释放。在发情周期第5天对两头奶牛进行输注，分别在54小时和69小时后出现前列腺素释放，随后黄体退化，并在输注后约五天出现发情。在第11天或第12天进行的输注，分别在147小时和120小时后出现前列腺素释放，随后在19天的发情周期后出现黄体溶解和发情。在第16天和第17天对两头奶牛进行输注，两头动物均在117小时后出现前列腺素释放。一个周期延长，而另一个周期持续时间正常。在第一次前列腺素激增后第20天进行输注的一头奶牛，其周期长度为26天，而在第20天进行输注的另一头奶牛未受影响，因为在输注时黄体溶解基本完成。在第5天进行输注的一头动物对碘输注无反应。然而，在这头动物中，黄体在输注前未完全发育。从这项研究可以得出结论：1）在分泌孕酮的黄体发育后进行宫内碘输注，会在三到六天内导致前列腺素释放，随后出现黄体溶解；2）在所有情况下，黄体溶解均与前列腺素F释放有关，其数量级和持续时间与正常黄体溶解期间相同。关键词：前列腺素释放；孕酮；奶牛；发情周期；碘输注
