刚地弓形虫的分离与保存
Isolation and preservation of Toxoplasma gondii.
作者信息
Kwantes W, Fleck D G, Payne R A
出版信息
J Clin Pathol. 1967 May;20(3):286-9. doi: 10.1136/jcp.20.3.286.
Abstract
Thirteen isolations of Toxoplasma gondii were made from 110 specimens of various human tissues and tissue fluids. Twelve of these were from lymph nodes and one from a specimen of cerebrospinal fluid; all isolations were from cases with dye test titres of 1/512 or more. A description of the deep freeze method used to preserve the T. gondii strains isolated is included. It is hoped that attempts at isolation of this organism will be made more frequently in future.
摘要
从110份不同人体组织和组织液标本中分离出13株刚地弓形虫。其中12株来自淋巴结，1株来自脑脊液标本；所有分离株均来自染料试验效价为1/512或更高的病例。文中还介绍了用于保存所分离刚地弓形虫菌株的深度冷冻方法。希望今后能更频繁地尝试分离这种病原体。
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Isolation and preservation of Toxoplasma gondii.刚地弓形虫的分离与保存
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本文引用的文献
1
Preservation of cells in tissue culture by glycerol and dimethyl sulphoxide.通过甘油和二甲基亚砜在组织培养中保存细胞。
Nature. 1962 Feb 10;193:548-50. doi: 10.1038/193548a0.
2
[Acquired toxoplasmosis. (Study of 227 cases)].
Arch Fr Pediatr. 1960;17:281-331.
3
Repeated congenital infection with toxoplasma gondii.先天性弓形虫反复感染
Obstet Gynecol. 1963 Mar;21:318-29.
4
Pathogenetic aspects of human acquired toxoplasmosis.人类获得性弓形虫病的发病机制方面
Surv Ophthalmol. 1961 Dec;6:781-98.
5
Use of dimethyl sulphoxide for preservation of tissue culture cells by freezing.
Nature. 1962 Feb 10;193:550-2. doi: 10.1038/193550a0.
6
Dimethyl sulphoxide, an alternative to glycerol, for the low-temperature preservation of trypanosomes.二甲基亚砜，甘油的替代品，用于锥虫的低温保存。
Ann Trop Med Parasitol. 1961 Apr;55:93-6. doi: 10.1080/00034983.1961.11686023.
7
Glandular toxoplasmosis; a survey of 30 cases.腺型弓形虫病；30例病例调查
Lancet. 1958 Aug 23;2(7043):379-84. doi: 10.1016/s0140-6736(58)90109-0.
8
Preservation of Toxoplasma gondii by freezing.通过冷冻保存弓形虫。
J Parasitol. 1956 Aug;42(4 Section 1):408-13.
9
Prolonged storage of toxoplasma at -70 C.; a preliminary report.弓形虫在-70℃下的长期保存：初步报告。
Am J Clin Pathol. 1956 Mar;26(3):323-6. doi: 10.1093/ajcp/26.3_ts.323.
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