Knights S F, Ingram G I
J Clin Pathol. 1967 Jul;20(4):616-9. doi: 10.1136/jcp.20.4.616.
Deficiencies of factor VIII (in haemophilia) and factor IX (in Christmas disease) prolong the partial thromboplastin time. If normal plasma is treated with alumina, the factor VIII remains but the factor IX is removed and can subsequently be recovered by elution of the alumina. If a long partial thromboplastin time is found on investigating a male patient whose history suggests a life-long bleeding disorder, the plasma may be retested after adding either alumina-adsorbed normal plasma or eluate. If the patient's partial thromboplastin time is shortened (relative to the control) by adding adsorbed normal plasma the patient is likely to be a haemophiliac; but if it is shortened by adding eluate then he is likely to have Christmas disease. Practical details for carrying out these manoeuvres are given and experiments on the validity of the test described.
因子 VIII（血友病中）和因子 IX（克里斯马斯病中）的缺乏会延长部分凝血活酶时间。如果用氧化铝处理正常血浆，因子 VIII 会保留下来，但因子 IX 会被去除，随后可通过洗脱氧化铝将其回收。如果在对一名有终生出血性疾病病史的男性患者进行检查时发现部分凝血活酶时间延长，可在添加氧化铝吸附的正常血浆或洗脱液后对血浆进行重新检测。如果添加吸附的正常血浆后患者的部分凝血活酶时间（相对于对照）缩短，则该患者很可能是血友病患者；但如果添加洗脱液后时间缩短，那么他很可能患有克里斯马斯病。文中给出了进行这些操作的实际细节以及关于所述检测有效性的实验。
