一种用于凝血试验和凝血因子测定的微量方法。
A micromethod for clotting tests and coagulation factor assays.
作者信息
Exner T, Margolis J, Rickard K A
出版信息
Clin Lab Haematol. 1980;2(3):227-30. doi: 10.1111/j.1365-2257.1980.tb00827.x.
A simple microtechnique for carrying out partial thromboplastin time with kaolin tests with 2 microliter or less of test plasma is described. For single stage factor assays, less than 1 microliter of test solution may be used. Reagents and test plasma are loaded in sequence into a 10 microliter, long needle syringe and introduced into a micro test-tube immobilized in a water bath. The end-point is taken as a positive clearing of kaolin turbidity from the mixture while stirring. Correlation with normal techniques has been excellent.
本文描述了一种简单的微量技术，可使用2微升或更少的测试血浆进行高岭土部分凝血活酶时间测试。对于单阶段因子测定，可使用少于1微升的测试溶液。将试剂和测试血浆按顺序装入10微升的长针注射器中，然后引入固定在水浴中的微量试管中。终点定义为在搅拌时混合物中高岭土浊度的阳性清除。与常规技术的相关性非常好。
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