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关于用于非酯化脂肪酸(N.E.F.A.)测定的血样采集与处理的观察

Observations on the collection and handling of blood samples for N.E.F.A. estimation.

作者信息

Fairweather D V, Layton R

出版信息

J Clin Pathol. 1967 Jul;20(4):665-7. doi: 10.1136/jcp.20.4.665.

DOI:10.1136/jcp.20.4.665
PMID:5602585
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC473533/
Abstract

The effect upon the results of estimating plasma or serum non-esterified fatty acids (N.E.F.A.) assay by different methods of collecting blood samples and subsequent differences in sample storage and time of assay have been studied. Standardization of technique is advised and the use of chilled heparinized tubes, with separation of the sample within one hour of collection and assay on the day of collection is advocated.

摘要

研究了通过不同采血方法以及随后样本储存和检测时间的差异对血浆或血清非酯化脂肪酸(N.E.F.A.)检测结果的影响。建议对技术进行标准化,提倡使用预冷的肝素化试管,在采血后一小时内分离样本并在采血当天进行检测。

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Observations on the collection and handling of blood samples for N.E.F.A. estimation.关于用于非酯化脂肪酸(N.E.F.A.)测定的血样采集与处理的观察
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本文引用的文献

1
CHANGES IN SERUM NON-ESTERIFIED FATTY ACID LEVELS IN SPONTANEOUS AND IN OXYTOCIN INDUCED LABOUR.
J Obstet Gynaecol Br Commonw. 1965 Jun;72:408-15. doi: 10.1111/j.1471-0528.1965.tb01477.x.
2
SERUM NONESTERIFIED FATTY ACID LEVELS IN HUMAN PREGNANCY AS DETERMINED BY VARIOUS TITRATION PROCEDURES.通过各种滴定法测定的人类孕期血清非酯化脂肪酸水平。
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3
Effects of low-carbohydrate diet and diabetes mellitus on plasma concentrations of glucose, non-esterified fatty acid, and insulin during oral glucose-tolerance tests.口服葡萄糖耐量试验期间低碳水化合物饮食和糖尿病对血浆葡萄糖、非酯化脂肪酸及胰岛素浓度的影响。
Lancet. 1963 Apr 13;1(7285):790-4. doi: 10.1016/s0140-6736(63)91501-0.
4
A colorimetric method of determining unesterified fatty acids in plasma.一种测定血浆中未酯化脂肪酸的比色法。
Scand J Clin Lab Invest. 1958;10(4):407-14. doi: 10.3109/00365515809051246.
5
Determination of unesterified fatty acids in normal human plasma.正常人血浆中游离脂肪酸的测定。
Clin Chim Acta. 1958 Sep;3(5):443-9. doi: 10.1016/0009-8981(58)90037-8.
6
Unesterified fatty acid in human blood plasma. II. The transport function of unesterified fatty acid.人体血浆中的游离脂肪酸。II. 游离脂肪酸的转运功能。
J Clin Invest. 1957 Jun;36(6 Part 1):810-5. doi: 10.1172/JCI103486.
7
A relation between non-esterified fatty acids in plasma and the metabolism of glucose.血浆中非酯化脂肪酸与葡萄糖代谢之间的关系。
J Clin Invest. 1956 Feb;35(2):150-4. doi: 10.1172/JCI103259.
8
Effect of lipemia and heparin on free fatty acid content of rat plasma.脂血症和肝素对大鼠血浆游离脂肪酸含量的影响。
Proc Soc Exp Biol Med. 1954 Nov;87(2):312-5. doi: 10.3181/00379727-87-21366.
9
Calcium, phosphorus, and fatty acids in plasma stored at room temperature.室温下储存的血浆中的钙、磷和脂肪酸。
Lancet. 1966 Jul 23;2(7456):196-8. doi: 10.1016/s0140-6736(66)92478-0.