Department of Clinical Chemistry, University Medical Center Goettingen, Goettingen, Germany.
Clin Chem Lab Med. 2011 Sep;49(9):1473-7. doi: 10.1515/CCLM.2011.606. Epub 2011 May 24.
In this study the pre-analytical effects of sample storage on frequently used routine clinical chemistry assays were evaluated by comparing four different lithium heparin plasma separation tubes to a reference collection procedure.
Blood was collected from 20 healthy volunteers using plasma separation tubes from four different manufacturers together with manually separated plasma as reference. In total, 15 clinical chemistry parameters were determined at 0 h, 24 h, and 72 h. Samples were stored at 4°C. Statistical differences were evaluated using a generalized estimating equation regression model.
Significant differences could be demonstrated for almost every parameter when comparing the separation tubes to the reference collection system. The estimated maximum allowable storage time in the primary tube was considerably reduced using separation tubes, e.g., for glucose the maximum storage time was reduced from >72 h to 7-15 h, and for potassium from 60 h to 10-13 h, respectively.
These data indicate that sample storage in the primary tube using plasma separation tubes is associated with clinically relevant changes for certain parameters. Therefore, storing samples for retesting should be avoided when using plasma separation tubes, in particular for parameters susceptible to interference by erythrocyte or platelet contamination.
本研究通过比较四种不同的肝素锂血浆分离管与参考采集程序，评估了样本储存对常用常规临床化学检测的分析前影响。
使用来自四个不同制造商的血浆分离管从 20 名健康志愿者中采集血液，并将手动分离的血浆作为参考。总共在 0 h、24 h 和 72 h 时测定了 15 个临床化学参数。将样品储存在 4°C。使用广义估计方程回归模型评估统计学差异。
当将分离管与参考采集系统进行比较时，几乎每个参数都显示出显著差异。与参考采集系统相比，使用分离管显著降低了主要管中允许的最大储存时间，例如葡萄糖的最大储存时间从>72 h 缩短至 7-15 h，钾的最大储存时间从 60 h 缩短至 10-13 h。
这些数据表明，使用血浆分离管在主要管中储存样本与某些参数的临床相关变化有关。因此，在使用血浆分离管时，应避免将样本储存起来进行重新检测，特别是对于容易受到红细胞或血小板污染干扰的参数。
