Knöchel W, Kohnert-Stavenhagen E
Hoppe Seylers Z Physiol Chem. 1977 Jul;358(7):835-42. doi: 10.1515/bchm2.1977.358.2.835.
The content of globin coding sequences in nuclear RNA from chicken embryo red blood cells and chicken embryo brain was determined by hybridization with globin cDNA to be 0.27% for embryonic red blood cells and 0.001% for embryo brain, i.e. 0.37% of globin coding sequences relative to embryonic red blood cells. By transfusion of [3H]uridine labelled red blood cells it could be shown that the brain nuclear RNA preparation was contaminated by 0.41% RNA originating from blood cells. As this value is in the same range as the hybridization rate there is no evidence for globin transcripts in brain nuclear RNA. It has also not been possible to detect a short-lived globin mRNA precursor in pulse labelled brain nuclear RNA. In further experiments the RNAs were translated in an Ehrlich ascites cell-free system. No globin synthesis could be observed with brain RNA.
通过与珠蛋白互补DNA杂交来测定鸡胚红细胞和鸡胚脑细胞核RNA中珠蛋白编码序列的含量，结果表明，胚胎红细胞中该含量为0.27%，胚胎脑中为0.001%，即相对于胚胎红细胞，珠蛋白编码序列占0.37%。通过输注[³H]尿苷标记的红细胞，可以证明脑细胞核RNA制剂被0.41%源自血细胞的RNA污染。由于该值与杂交率处于同一范围，因此没有证据表明脑细胞核RNA中存在珠蛋白转录本。在脉冲标记的脑细胞核RNA中也未能检测到短寿命的珠蛋白mRNA前体。在进一步的实验中，这些RNA在艾氏腹水无细胞系统中进行翻译。用脑RNA未观察到珠蛋白合成。
