Cox K O, Baddams H, Evans A
Aust J Exp Biol Med Sci. 1977 Feb;55(1):27-37. doi: 10.1038/icb.1977.3.
The effects of the proteolytic enzyme bromelain (Br) on the antigenicity and immunogenicity of sheep and mouse red blood cells (RBC) have been investigated. The results presented support the previous claim that there are antigens present on Br RBC that are not present in an exposed form on untreated RBC and that Br RBC have lost some of the antigens present on the surface of normal RBC. The susceptibility of Br RBC to osmotic lysis was very similar to that of normal RBC, implying that the modified RBC were not more fragile than normal RBC. Injection of mice with Br mouse-RBC did not increase the unusually high "background" number of cells producing IgM antibodies against Br mouse-RBC and mice did not mount delayed-type hypersensitivity reactions against Br mouse-RBC, either before or after sensitizing injections of Br mouse-RBC. However, mouse-RBC and Br mouse-RBC elicited similar antibody responses in rabbits and guinea pigs. Although mice appeared unresponsive to Br mouse-RBC injections, delayed-type hypersensitivity responses and antibody production in primary and secondary responses were of similar levels irrespective of whether sheep-RBC or Br sheep-RBC were used as immunogens. From these studies it appears that mice have B-cells producing antibodies against the "new" antigens on Br mouse-RBC, but there are no T-cells that respond to these antigens by way of "helper" activity in antibody production or by way of cell-mediated immune reactions.
已研究了蛋白水解酶菠萝蛋白酶（Br）对绵羊和小鼠红细胞（RBC）抗原性和免疫原性的影响。所呈现的结果支持了先前的说法，即Br处理的红细胞上存在一些未处理红细胞上未以暴露形式存在的抗原，并且Br处理的红细胞已失去了正常红细胞表面存在的一些抗原。Br处理的红细胞对渗透裂解的敏感性与正常红细胞非常相似，这意味着经过修饰的红细胞并不比正常红细胞更脆弱。给小鼠注射Br处理的小鼠红细胞并没有增加产生抗Br处理的小鼠红细胞IgM抗体的异常高的“背景”细胞数量，并且在致敏注射Br处理的小鼠红细胞之前或之后，小鼠对Br处理的小鼠红细胞都没有产生迟发型超敏反应。然而，小鼠红细胞和Br处理的小鼠红细胞在兔和豚鼠中引发了相似的抗体反应。尽管小鼠似乎对注射Br处理的小鼠红细胞无反应，但无论使用绵羊红细胞还是Br处理的绵羊红细胞作为免疫原，初次和二次反应中的迟发型超敏反应和抗体产生水平相似。从这些研究看来，小鼠具有产生针对Br处理的小鼠红细胞上“新”抗原的抗体的B细胞，但没有通过抗体产生中的“辅助”活性或通过细胞介导的免疫反应对这些抗原作出反应的T细胞。
