Mean F, Pellaton M, Magrini G
Clin Chim Acta. 1977 Oct 1;80(1):171-80. doi: 10.1016/0009-8981(77)90276-5.
Saturation studies on sex hormone binding globulin (SHBG) were performed with increasing levels of the three steroids: dihydrotestosterone (DHT), testosterone (T) and oestradiol (E2) in normal female sera. The curves obtained showed the clearly increasing SHBG affinity for E2, T and DHT, respectively. With T as saturating ligand, the mean values of SHBG binding capacity in normal and pathological sera, expressed as 10(-8) M SHBG were, for normal women, 5.87 +/- 0.2, for normal men, 3.69 +/- 0.1 and for hirsute women, 5.05 +/- 0.2. In hirsutism, SHBG levels were measured together with T and DHT levels. Hyperandrogenia was often better reflected by the T/SHBG ratio than by T levels alone. SHBG binding capacity, measured in pregnancy from the 7th to the 40th week, increased progressively as far as the 30th week. Values up to 5 times those of the mean value of the cycle were observed at the end of pregnancy. Variations in DHT, T and E2 binding as functions of SHBG concentrations, were also studied by adding a constant amount of each steroid to increasing SHBG concentrations in serum. DHT showed the greatest binding capacity, followed by T and then E2. Since the unbound fractions of T and DHT are believed to be biologically active, and since T and DHT are more sensitive than E2 to SHBG variations, the results obtained further support the hypothesis that SHBG plays an important role in the sex steroids balance.
采用正常女性血清中三种甾体激素（双氢睾酮（DHT）、睾酮（T）和雌二醇（E2））水平递增的方式，对性激素结合球蛋白（SHBG）进行饱和度研究。所得曲线分别清晰显示了SHBG对E2、T和DHT的亲和力递增。以T作为饱和配体时，正常和病理血清中SHBG结合能力的平均值（以10^(-8) M SHBG表示），正常女性为5.87±0.2，正常男性为3.69±0.1，多毛女性为5.05±0.2。在多毛症患者中，同时测定了SHBG水平以及T和DHT水平。雄激素过多症通常通过T/SHBG比值比单独的T水平能更好地反映出来。在妊娠第7周至第40周期间测量SHBG结合能力，直至第30周其逐渐升高。在妊娠末期观察到的值高达月经周期平均值的5倍。通过向血清中递增的SHBG浓度添加恒定剂量的每种甾体激素，还研究了DHT、T和E2结合随SHBG浓度的变化情况。DHT显示出最大的结合能力，其次是T，然后是E2。由于T和DHT的未结合部分被认为具有生物活性，并且由于T和DHT比E2对SHBG变化更敏感，所获得的结果进一步支持了SHBG在性甾体激素平衡中起重要作用这一假说。
