Fiser P S, Macpherson J W
J Dairy Sci. 1977 Aug;60(8):1301-3. doi: 10.3168/jds.S0022-0302(77)84026-5.
The ability of the cryoprotective solution containing dimethylsulphoxide in combination with yolk extract (DYG 550) to protect mouse embryos at ultra low temperatures is reported. Of the embryos of 46.1% frozen to -196 C at various rates formed blastocysts in vitro compared to 41.2% and 23.2% survivals protected by 1M dimethylsulphoxide and 1M glycerol.
