Rinehart J J, Boulware T
J Lab Clin Med. 1977 Oct;90(4):737-43.
The functions of microfilaments and microtubules in human monocytes were examined by exposing peripheral blood monocytes to cytochalasin B, a microfilament inhibitor, and to colchicine, an antitubulin. Cytochalasin B, 10 microgram/ml, inhibited all monocyte functions tested: chemotaxis was reduced by 96% of control levels (p less than 0.01), random mobility by 88% (p less than 0.01), candidacidal activity by 57% (p less than 0.01), and phagocytosis by 34% (p less than 0.01). Monocyte adherence was also significantly reduced (by 65%). Colchicine, 10 microgram/ml, significantly reduced monocyte chemotaxis by 39% (p less than 0.01) and random mobility by 59% (p less than 0.01), but phagocytosis and candidacidal activity were not diminished at 0.1 to 100 microgram/ml. Monocyte adherence was modestly decreased (46%) by 10 microgram/ml of colchicine. Thus, cytochalasin B, a microfilament inhibitor, reduced all five functions tested. In contrast, colchicine, an antitubulin, inhibited only chemotaxis, random mobility, and adherence. These data suggest that in monocytes (1) microfilaments are the structural element of cell membrane and cytoplasm required for most cellular functions and (2) microtubules facilitate membrane surface interaction in chemotaxis, random mobility, and adherence.
通过将外周血单核细胞暴露于微丝抑制剂细胞松弛素B和抗微管蛋白秋水仙碱，研究了微丝和微管在人单核细胞中的功能。10微克/毫升的细胞松弛素B抑制了所有测试的单核细胞功能：趋化性降低至对照水平的96%（p<0.01），随机运动降低88%（p<0.01），杀念珠菌活性降低57%（p<0.01），吞噬作用降低34%（p<0.01）。单核细胞黏附也显著降低（65%）。10微克/毫升的秋水仙碱使单核细胞趋化性显著降低39%（p<0.01），随机运动降低59%（p<0.01），但在0.1至100微克/毫升时吞噬作用和杀念珠菌活性未降低。10微克/毫升的秋水仙碱使单核细胞黏附适度降低（46%）。因此，微丝抑制剂细胞松弛素B降低了所有测试的五项功能。相比之下，抗微管蛋白秋水仙碱仅抑制趋化性、随机运动和黏附。这些数据表明，在单核细胞中：（1）微丝是大多数细胞功能所需的细胞膜和细胞质的结构成分；（2）微管在趋化性、随机运动和黏附中促进膜表面相互作用。
