Athlin L, Domellof L, Norberg B O
Lymphology. 1986 Sep;19(3):130-5.
The effect of microtubule antagonist (MA) colchicine and its two non-MA derivatives beta-lumicolchicine and gamma-lumicolchicine on the phagocytosis of yeast cells by glass-adherent monocytes were studied with a fluorescence quenching technique which allowed a distinction between the adherence and engulfment phases of phagocytosis. The engulfment of yeast cells was depressed by colchicine (p = 0.031) but not by the lumicolchicines. The adherence of yeast cells to the monocytes, on the other hand, was depressed by gamma-lumicolchicine (p = 0.028). The colchicine effect suggests that cytoplasmic microtubules play a role in the engulfment process whereas the lumicolchicine effect suggests a role of nucleoside transport over the cell membrane in the adherence process of phagocytosis.
采用荧光猝灭技术研究了微管拮抗剂（MA）秋水仙碱及其两种非MA衍生物β-光秋水仙碱和γ-光秋水仙碱对玻璃黏附单核细胞吞噬酵母细胞的影响，该技术能够区分吞噬作用的黏附阶段和吞噬阶段。秋水仙碱可抑制酵母细胞的吞噬作用（p = 0.031），但光秋水仙碱无此作用。另一方面，γ-光秋水仙碱可抑制酵母细胞与单核细胞的黏附（p = 0.028）。秋水仙碱的作用表明细胞质微管在吞噬过程中发挥作用，而光秋水仙碱的作用表明核苷转运在吞噬作用的黏附过程中通过细胞膜发挥作用。
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