González-Menció F, Murphy B D, Manns J
Prostaglandins. 1977 Sep;14(3):535-42. doi: 10.1016/0090-6980(77)90267-2.
Henderson and McNatty (Prostaglandins 9:779, 1975) proposed that LH from the preovulatory LH surge attached to receptors on luteal cells and that this attachment might protect the early corpus luteum from PGF2alpha induced luteolysis. To test this hypothesis, experiments were performed on heifers at day 10-12 of the cycle. Both jugular veins were catheterized and infusions of either saline (0.64 ml/min) or LH-NIH-B9 (10 microgram/min; 0.64 ml/min) were given. Saline infusions were from 0-12 h; LH infusions were for 10 h and were preceded by a 2 h saline infusion. All animals were given 25 mg PGF2alpha im at 6 h (6 h into the saline infusion and 4 h into the LH infusion). Blood samples were taken at 0.5 h, 1 h and 4 h intervals from 0-12h, 13-18 h and 12-42 h respectively. Serum was assayed for LH and progesterone by radioimmunoassay methods. Two animals received saline and two received LH in each experiment. Each treatment was replicated 6 times. LH infusion resulted in a mean serum LH of 75 ng/ml compared to 0.90 ng/ml in saline infused animals. This elevation of LH did not alter PGF2alpha induced luteolysis as indicated by decline in serum progesterone. This experiment does not support the hypothesis that the newly formed corpus luteum is resistant to PGF2alpha because of protection afforded by the proestrus LH surge.
亨德森和麦克纳蒂（《前列腺素》9:779，1975年）提出，排卵前促黄体生成素高峰产生的促黄体生成素附着在黄体细胞的受体上，这种附着可能会保护早期黄体免受前列腺素F2α诱导的黄体溶解。为了验证这一假设，在周期第10 - 12天对小母牛进行了实验。将两条颈静脉插管，分别输注生理盐水（0.64毫升/分钟）或促黄体生成素 - NIH - B9（10微克/分钟；0.64毫升/分钟）。生理盐水输注时间为0 - 12小时；促黄体生成素输注时间为10小时，在输注前先有2小时的生理盐水输注。所有动物在6小时时（生理盐水输注6小时、促黄体生成素输注4小时）肌肉注射25毫克前列腺素F2α。分别在0 - 12小时、13 - 18小时和12 - 42小时，每隔0.5小时、1小时和4小时采集血样。通过放射免疫分析方法测定血清中的促黄体生成素和孕酮。每个实验中有两只动物接受生理盐水输注，两只接受促黄体生成素输注。每种处理重复6次。与输注生理盐水的动物血清促黄体生成素平均为0.90纳克/毫升相比，输注促黄体生成素导致血清促黄体生成素平均为75纳克/毫升。如血清孕酮下降所示，促黄体生成素的这种升高并未改变前列腺素F2α诱导的黄体溶解。该实验不支持以下假设，即由于发情前期促黄体生成素高峰提供的保护作用，新形成的黄体对前列腺素F2α具有抗性。
