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生长中的小鼠卵母细胞的体外培养。

In vitro culture of growing mouse oocytes.

作者信息

Baran M M, Bachvarova R

出版信息

J Exp Zool. 1977 Nov;202(2):283-9. doi: 10.1002/jez.1402020219.

DOI:10.1002/jez.1402020219
PMID:562922
Abstract

A method is described for in vitro culture of naked growing mouse oocytes for at least four days. Tests for metabolic function indicate that the oocytes increase in volume, accumulate glucose-6-phosphate dehydrogenase, and maintain a steady level of 3H-uridine and 3H-leucine incorporation.

摘要

描述了一种体外培养裸生长小鼠卵母细胞至少四天的方法。代谢功能测试表明,卵母细胞体积增大,积累葡萄糖-6-磷酸脱氢酶,并维持3H-尿苷和3H-亮氨酸掺入的稳定水平。

相似文献

1
In vitro culture of growing mouse oocytes.生长中的小鼠卵母细胞的体外培养。
J Exp Zool. 1977 Nov;202(2):283-9. doi: 10.1002/jez.1402020219.
2
[In vitro culture of oocytes from growing mice: behavior in defined medium and in the presence of follicular cells].[生长中小鼠卵母细胞的体外培养:在限定培养基及卵泡细胞存在情况下的行为]
Boll Soc Ital Biol Sper. 1980 Apr 30;56(8):826-32.
3
Development of naked growing mouse oocytes in vitro.
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4
Bovine blastocyst development rate in vitro is influenced by selection of oocytes by brillant cresyl blue staining before IVM as indicator for glucose-6-phosphate dehydrogenase activity.体外成熟前通过灿烂甲酚蓝染色选择卵母细胞作为葡萄糖-6-磷酸脱氢酶活性指标,会影响牛体外囊胚发育率。
Theriogenology. 2005 May;63(8):2194-205. doi: 10.1016/j.theriogenology.2004.09.050.
5
Translation and stability of ovalbumin messenger RNA injected into growing oocytes and fertilized ova of mice.注射到生长中的小鼠卵母细胞和受精卵中的卵清蛋白信使核糖核酸的翻译与稳定性
J Embryol Exp Morphol. 1984 Dec;84:91-103.
6
Cytochemical determination of glucose-6-phosphate dehydrogenase activity in isolated mouse oocytes.
Acta Histochem Suppl. 1984;29:149-53.
7
[Influence of follicular cells on energy metabolism of oocytes in growing mice].
Boll Soc Ital Biol Sper. 1982 Jul 30;58(14):900-6.
8
Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer.通过灿烂甲酚蓝染色选择发育能力良好的卵母细胞可提高牛核移植后的囊胚发育率。
Theriogenology. 2007 Jan 15;67(2):341-5. doi: 10.1016/j.theriogenology.2006.08.006. Epub 2006 Sep 26.
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rRNA accumulation and protein synthetic patterns in growing mouse oocytes.生长中的小鼠卵母细胞中的核糖体RNA积累和蛋白质合成模式
J Exp Zool. 1982 May 1;220(3):361-70. doi: 10.1002/jez.1402200311.
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Biochemical studies of growing mouse oocytes: preparation of oocytes and analysis of glucose-6-phosphate dehydrogenase and lactate dehydrogenase activities.
Dev Biol. 1975 Aug;45(2):211-20. doi: 10.1016/0012-1606(75)90061-5.