Salustri A, Martinozzi M
Boll Soc Ital Biol Sper. 1980 Apr 30;56(8):826-32.
To approach the problem of the possible role of granulosa cells in oocyte growth we utilized different systems for the culture in defined medium, isolated oocyte culture in "conditioned medium" from follicle culture, isolated oocyte-follicle cells coculture. Tests for metabolic functions indicate that the oocytes maintain the same steady level of 3H leucine incorporation and fail to grow in all of the culture systems considered. In isolated follicle culture, however, granulosa cell enclosed oocytes increase in volume. The results support the hypothesis that junctional association between oocytes and granulosa cells must be maintained to promote oocytes growth "in vitro".
为探讨颗粒细胞在卵母细胞生长中可能发挥的作用这一问题，我们在限定培养基中利用不同系统进行培养，包括在卵泡培养的“条件培养基”中进行分离的卵母细胞培养、分离的卵母细胞 - 卵泡细胞共培养。代谢功能测试表明，在所有所考虑的培养系统中，卵母细胞的³H亮氨酸掺入水平保持相同的稳定状态且未能生长。然而，在分离的卵泡培养中，被颗粒细胞包裹的卵母细胞体积增大。这些结果支持这样的假说，即卵母细胞与颗粒细胞之间的连接关联必须得以维持，以促进“体外”卵母细胞生长。
