Liashko O G
Arkh Anat Gistol Embriol. 1977 Oct;73(10):86-91.
The study was performed during the period close to equinox in 236 male mice C57 Bl strain, sacrificed at equal intervals during 5 days. In paraffin sections, 5--6 mcm thick, stained after Dominici-Kedrovsky, with additional application of PAS-reaction and alcian blue, the cell height and width in the small intestine border enterocyte zone were determined. Calculation of autocorrelative function with the following determination of spectral density, infradian, circadian and ultradian biorhythmical components were estimated, as well as their changes under the influence of feeding regime inversion. At evening feeding, the greatest width of the brush border coinsides the beginning and the end of the digestion period. The width of the dark apical zone, where mitochondria are concentrated, synchronously changes at the end of digestion with the brush border, and then- with the cell basal zone. At morning feeding, by the degree of circadian phase change, the most liable are the indices reflecting organoid status, which are closely involved in absorbtion processes. Ultradian rhythm produces, on the background of circadian changes, additional raisis and abatements. Spectral composition, under inadequate feeding, is demonstrated to become more complex, mainly at the expense of ultradian rhythms, that speaks of their importance for the processes of adaptive rhythmical rearrangement.
该研究在接近春分的时期对236只C57 Bl品系雄性小鼠进行,在5天内以相等的间隔处死。在5 - 6微米厚的石蜡切片中,按照多米尼奇 - 凯德罗夫斯基法染色,并额外进行PAS反应和阿尔辛蓝染色,测定小肠边缘肠上皮细胞区的细胞高度和宽度。计算自相关函数,并随后确定光谱密度,估计亚日、昼夜和超日生物节律成分,以及它们在进食制度颠倒影响下的变化。在夜间喂食时,刷状缘的最大宽度与消化期的开始和结束相吻合。线粒体集中的深色顶端区域的宽度在消化结束时与刷状缘同步变化,然后与细胞基部区域同步变化。在早晨喂食时,根据昼夜节律阶段变化的程度,最容易变化的是反映细胞器状态的指标,这些指标与吸收过程密切相关。在昼夜变化的背景下,超日节律产生额外的升高和降低。结果表明,在喂食不足的情况下,光谱组成会变得更加复杂,主要是以牺牲超日节律为代价,这表明它们对适应性节律重排过程很重要。