Curran K L, Kupchella C E, Tamburro C H
Cancer. 1977 Dec;40(6):3050-3. doi: 10.1002/1097-0142(197712)40:6<3050::aid-cncr2820400643>3.0.co;2-p.
Glycosaminoglycans extracted from 24-hour urine specimens from patients with hepatic angiosarcoma and from normal/controls were separated as cetylpyridinium complexes into "hyaluronic acid," "chondroitin sulfate," and "heparin" fractions, then further separated and characterized by anion-exchange chromatography and hyaluronidase susceptibility. The chromatographic pattern of the urinary chondroitin sulfate fraction in patients with angiosarcoma of the liver differed from those of controls in that there was a relative increase in the total amount of uronic acid in a hyaluronidase-resistant fraction and a decrease in a fraction susceptible to hyaluronidase digestion. These changes appeared to become more pronounced with advancing disease. Chromatographic patterns and determinations of hyaluronidase susceptibility indicated that the resistant fraction was heparan sulfate and that the susceptible fraction was chondroitin-4-sulfate and/or chondroitin-6-sulfate.
从肝血管肉瘤患者以及正常对照者的24小时尿液标本中提取的糖胺聚糖，以十六烷基吡啶络合物的形式分离为“透明质酸”、“硫酸软骨素”和“肝素”组分，然后通过阴离子交换色谱法和对透明质酸酶的敏感性进行进一步分离和表征。肝血管肉瘤患者尿液中硫酸软骨素组分的色谱图谱与对照组不同，在于抗透明质酸酶消化的组分中糖醛酸总量相对增加，而对透明质酸酶消化敏感的组分中糖醛酸总量减少。随着疾病进展，这些变化似乎变得更加明显。色谱图谱和对透明质酸酶敏感性的测定表明，抗酶解组分是硫酸乙酰肝素，而敏感组分是硫酸软骨素-4-硫酸盐和/或硫酸软骨素-6-硫酸盐。
