人尿糖胺聚糖的乙醇分级分离

Fractionation with ethanol of human urinary glycosaminoglycans.

作者信息

Endo M, Namiki O, Yosizawa Z

出版信息

Tohoku J Exp Med. 1980 May;131(1):23-8. doi: 10.1620/tjem.131.23.

DOI:10.1620/tjem.131.23

Abstract

Crude glycosaminoglycans (GAG) separated from normal human urine by cetylpyridinium chloride-precipitation were fractionated with ethanol. The resulting fractions were then examined by electrophoresis on cellulose acetate membranes before and after enzymatic digestions and nitrous acid treatment. Analytical data for these fractions and the electrophoretograms suggested that ethanol-fractionation was useful to separate roughly chondroitin sulfate A, C-type GAG and heparan sulfate in human urine.

摘要

通过十六烷基氯化吡啶沉淀从正常人尿液中分离出的粗糖胺聚糖（GAG）用乙醇进行分级分离。然后，对所得级分在酶消化和亚硝酸处理前后在醋酸纤维素膜上进行电泳分析。这些级分的分析数据和电泳图谱表明，乙醇分级分离有助于大致分离人尿液中的硫酸软骨素A、C型GAG和硫酸乙酰肝素。