Doyle J M, Schachner M, Davidson R L
J Cell Physiol. 1977 Nov;93(2):197-203. doi: 10.1002/jcp.1040930205.
In a survey of the expression on cultured mouse cells of the cell surface antigen known as nervous system antigen-3 (NS-3), it was found that RAG, a renal adenocarcinoma line, expressed that antigen. It was also observed that 3T3, a fibroblast line of unknown tissue origin, expressed NS-3. Cells of these two lines were hybridized with cells of two mouse L cell lines that did not express NS-3. Four hybrid clones were tested for both the 3T3 X L cell cross and the RAG X L cell cross, and all the hybrids were found to be NS-3 positive. All the hybrids had at least 40% as much activity as the NS-3 positive parent. Of the four parental mouse cell lines used, only 3T3 expressed Thy-1.2 antigen on the cell surface. In contrast to the continued expression of NS-3 on hybrid cells, Thy-1.2 antigen was not detectable on two clones of 3T3 X L cell hybrids that were tested.
在一项关于培养的小鼠细胞中称为神经系统抗原-3（NS-3）的细胞表面抗原表达情况的调查中，发现肾腺癌系RAG表达了该抗原。还观察到来源不明的成纤维细胞系3T3也表达NS-3。将这两个细胞系的细胞与两种不表达NS-3的小鼠L细胞系的细胞进行杂交。对3T3×L细胞杂交和RAG×L细胞杂交产生的四个杂交克隆进行了检测，发现所有杂交细胞均为NS-3阳性。所有杂交细胞的活性至少是NS-3阳性亲本的40%。在所使用的四个亲本小鼠细胞系中，只有3T3在细胞表面表达Thy-1.2抗原。与杂交细胞持续表达NS-3相反，在检测的3T3×L细胞杂交的两个克隆中未检测到Thy-
