Dei T, Masuko K, Ishii H, Makita T, Tachibana T
J Natl Cancer Inst. 1986 Aug;77(2):511-9.
Spontaneous mammary carcinoma cells of C3H/He mice were fused with syngeneic L-cells, and two types of hybrid cell clones were obtained. In group A, hybrid cells showed contact inhibition, and parental H-2k and L-antigens were well expressed. Metacentric chromosomes of L-cell origin and estrogen dependency were also well preserved in this group. However, in group B, hybrid cells proliferated to pile up, and the expression of parental antigens, H-2 and L-cell antigens, was strongly suppressed. But, mouse mammary tumor antigens (MM-antigens), which were expressed on ascites mammary tumor cells with hypotetraploidy of C3H/He origin and which were not expressed on both parent cells, were newly expressed. The number of metacentric chromosomes was decreased, and estrogen dependency was lost in this group. The relationship between the expression of MM-antigens and that of H-2 antigens was reciprocal, and tumorigenicity was independent of cellular behavior in vitro and of MM-antigen expression. MM-antigen-positive hybrid cell clones were frequently obtained when tumor cells were fused with metaphase-rich L-cells.
将C3H/He小鼠的自发性乳腺癌细胞与同基因L细胞融合，获得了两种类型的杂交细胞克隆。在A组中，杂交细胞表现出接触抑制，亲本H-2k和L抗原表达良好。L细胞来源的中着丝粒染色体和雌激素依赖性在该组中也得到了很好的保留。然而，在B组中，杂交细胞增殖堆积，亲本抗原H-2和L细胞抗原的表达受到强烈抑制。但是，在具有C3H/He来源的亚四倍体的腹水型乳腺肿瘤细胞上表达且在两个亲本细胞上均不表达的小鼠乳腺肿瘤抗原（MM抗原）被新表达。该组中着丝粒染色体的数量减少，雌激素依赖性丧失。MM抗原的表达与H-2抗原的表达呈负相关，致瘤性与体外细胞行为和MM抗原表达无关。当肿瘤细胞与富含中期的L细胞融合时，经常获得MM抗原阳性的杂交细胞克隆。
