Nagelberg D B, Nagy Z M
Pharmacol Biochem Behav. 1977 Nov;7(5):435-41. doi: 10.1016/0091-3057(77)90211-8.
Utilizing a dosage of cycloheximide which was found to inhibit cerebral protein synthesis by almost 90% after injection, separate groups of 13-day-old mice received either cycloheximide or saline followed by 0 (control), 15, or 25 training trials in a discriminated shock-escape T-maze. Twenty-four hr later, each mouse was treated with cycloheximide or saline and tested for retention by an additional 25 trails in the T-maze. As reflected by correct choice-point turns, the results suggest that whereas salinetreated mice demonstrated reliable retention of prior learning, cycloheximide treated mice exhibited memory impairment; cycloheximide per se had no effect on performance during either original training or retest. A final experiment indicated that this memory impairment was not due to cycloheximide's general debilitating side effects at the time of retention testing. Taken together, these data suggest that protein synthesis inhibition during training impaired consolidation and/or retrieval processes involved in memory. The biochemical and behavioral effects following cycloheximide injection in 13-14-day-old mice in the present study parallel those reported with adult animals and lend indirect support to the hypothesis that the 24-hr memory capacity exhibited by these young mice reflects the early functioning of those processes involved in adult long-term memory.
利用一种剂量的放线菌酮，该剂量在注射后被发现可抑制大脑蛋白质合成近90%，将13日龄的小鼠分成不同组，每组小鼠分别接受放线菌酮或生理盐水注射，随后在一个有辨别性电击逃避的T型迷宫中进行0次（对照）、15次或25次训练试验。24小时后，每只小鼠再次接受放线菌酮或生理盐水处理，并在T型迷宫中通过额外的25次试验测试记忆保持情况。从正确的选择点转向情况可以看出，结果表明，用生理盐水处理的小鼠表现出对先前学习的可靠记忆保持，而用放线菌酮处理的小鼠则表现出记忆损伤；放线菌酮本身在初始训练或重新测试期间对表现没有影响。最后一项实验表明，这种记忆损伤并非由于在记忆保持测试时放线菌酮产生的一般衰弱性副作用所致。综合来看，这些数据表明训练期间的蛋白质合成抑制损害了记忆中涉及的巩固和/或提取过程。本研究中对13 - 14日龄小鼠注射放线菌酮后的生化和行为效应与成年动物的相关报道相似，这间接支持了以下假设：这些幼鼠表现出的24小时记忆能力反映了参与成年长期记忆的那些过程的早期功能。
