Boyns A R, Hardwicke J
Immunology. 1968 Mar;14(3):367-78.
Twenty-three rabbits were injected over long periods of time with isotopically labelled bovine serum albumin. Varying injection schedules and primary and secondary responses were studied, but all schedules were designed to preclude the presence of free circulating antibody at any time. All animals became tolerant of injected antigen after a period of 60–80 days. During the intervening period (from 10 to 60 days) large amounts of antigen—antibody complex were shown to be circulating. Proteinuria was intermittent in all animals. Histological examination of the kidneys from 50 days on showed only very minor evidence of renal damage; no animal developed chronic renal disease.
23只兔子长时间注射了同位素标记的牛血清白蛋白。研究了不同的注射方案以及初次和二次反应，但所有方案的设计目的都是在任何时候都排除游离循环抗体的存在。60 - 80天后，所有动物都对注射的抗原产生了耐受性。在这期间（10至60天），大量抗原 - 抗体复合物在循环中出现。所有动物都出现间歇性蛋白尿。从第50天开始对肾脏进行组织学检查，仅显示出非常轻微的肾损伤迹象；没有动物发展为慢性肾病。
