Robbins E, Jentzsch G, Micali A
J Cell Biol. 1968 Feb;36(2):329-39. doi: 10.1083/jcb.36.2.329.
Progression of the HeLa cell through its life cycle is accompanied by centriolar replication and pericentriolar changes that are in synchrony with DNA synthesis and mitosis. The first signs of preparation for replication occur during G(1) at which time the two orthogonal centrioles separate. Replication by budding begins at/or near the initiation of DNA synthesis and is completed by G(2). Pericentriolar changes which probably are causally related to spindle tubule formation occur at this time and include the appearance of vesicles, electron-opaque bodies, and an amorphous pericentriolar halo. These phenomena begin to disappear by late prophase, and the remainder of mitosis manifests decreasing centriolar and pericentriolar activity.
海拉细胞在其生命周期中的进展伴随着中心粒复制和中心粒周围的变化,这些变化与DNA合成和有丝分裂同步。复制准备的最初迹象出现在G(1)期,此时两个相互垂直的中心粒分离。通过出芽进行的复制在DNA合成开始时或附近开始,并在G(2)期完成。此时发生的中心粒周围变化可能与纺锤体微管形成有因果关系,包括囊泡、电子不透明体和无定形中心粒周围晕的出现。这些现象在前期后期开始消失,有丝分裂的其余阶段表现为中心粒和中心粒周围活动的减少。