Poretz R D, Goldstein I J
Immunology. 1968 Feb;14(2):165-74.
A comparison of the behaviour of the concanavalin A—polysaccharide and antibody—antigen systems with regard to various parameters of turbidity was conducted. It was demonstrated that in common with antibody—antigen systems, the reaction of concanavalin A with polysaccharides: (1) shows the ratio which yields the highest rate of development of turbidity is in the moderate concanavalin A excess region, (2) yields a series of apparent equivalence curves by turbidimetry which differ from the equivalence curves obtained by the quantitative precipitin method, and (3) shows a decrease in the rate of turbidity development in the presence of hapten inhibitor. In addition it was demonstrated that the degree of inhibition of the maximum constant turbidity caused by hapten inhibitors was identical to the degree of inhibition as determined by the amount of convanavalin A which precipitated. It was also shown that the quantitative estimation of inhibition (as determined turbidimetrically) at any time during the formation of turbidity yielded results which were directly proportional to those obtained by the quantitative precipitin method.
对伴刀豆球蛋白A-多糖体系和抗体-抗原体系在各种浊度参数方面的行为进行了比较。结果表明，与抗体-抗原体系一样，伴刀豆球蛋白A与多糖的反应：（1）显示出产生最高浊度发展速率的比例处于伴刀豆球蛋白A适度过量区域；（2）通过比浊法产生一系列表观等价曲线，这些曲线与通过定量沉淀素法获得的等价曲线不同；（3）在存在半抗原抑制剂的情况下，浊度发展速率降低。此外，还证明了半抗原抑制剂对最大恒定浊度的抑制程度与通过沉淀的伴刀豆球蛋白A的量所确定的抑制程度相同。还表明，在浊度形成过程中的任何时候，通过比浊法对抑制作用的定量估计（所确定的）产生的结果与通过定量沉淀素法获得的结果成正比。
