Doyle R J, Birdsell D C
J Bacteriol. 1972 Feb;109(2):652-8. doi: 10.1128/jb.109.2.652-658.1972.
Interactions between concanavalin A and cell wall digests of Bacillus subtilis 168 resulted in insoluble complexes as observed by double gel diffusion, turbidity, and analysis of the precipitate. The macromolecular constituent of the cell walls complexing with concanavalin A was the polyglucosylglycerol phosphate teichoic acid. The complex exhibited two pH optima: 3.1 and 7.4. The complex could be dissociated by saccharides which bind to concanavalin A. In contrast to concanavalin A-neutral polysaccharide complexes, formation of the concanavalin A-wall complex was inhibited by salts. It was subsequently shown that salts induce conformational changes in cell wall digests. The data suggested that for complex formation to occur a rigid rod conformation in the glucosylated teichoic acid is probably necessary. Concanavalin A can be used as a probe to study structural features of bacterial cell walls.
刀豆球蛋白A与枯草芽孢杆菌168细胞壁消化产物之间的相互作用，通过双向凝胶扩散、浊度测定以及沉淀物分析观察到会形成不溶性复合物。与刀豆球蛋白A形成复合物的细胞壁大分子成分是聚葡糖基甘油磷酸壁酸。该复合物表现出两个pH最佳值：3.1和7.4。该复合物可被与刀豆球蛋白A结合的糖类解离。与刀豆球蛋白A-中性多糖复合物不同，盐会抑制刀豆球蛋白A-细胞壁复合物的形成。随后表明，盐会诱导细胞壁消化产物的构象变化。数据表明，为了形成复合物，糖基化壁酸中可能需要刚性棒状构象。刀豆球蛋白A可作为研究细菌细胞壁结构特征的探针。
