深红红螺菌固氮酶制剂对异腈、乙炔和氰离子的还原动力学及抑制剂的影响
The kinetics of the reduction of isocyanides, acetylenes and the cyanide ion by nitrogenase preparation from Azotobacter chroococcum and the effects of inhibitors.
作者信息
Kelly M
出版信息
Biochem J. 1968 Mar;107(1):1-6. doi: 10.1042/bj1070001.
Abstract
- Nitrogen-fixing preparations from Azotobacter chroococcum reduced substrates with the following K(m) values: methyl isocyanide, 1.8x10(-4)m; ethyl isocyanide, 2.5x10(-2)m; cyanide ion, 1.4x10(-3)m; acetylene, 1.2x10(-4)m. 2. Nitrogen, carbon monoxide or hydrogen competitively inhibited isocyanide reduction with the following K(i) values: hydrogen, 1.3x10(-3)m; carbon monoxide, 6.8x10(-6)m; nitrogen, 4.3x10(-4)m. 3. Living nitrogen-fixing bacteria, and isolated clover nodules, formed methane from methyl isocyanide. 4. These results are discussed in relation to other work and possible mechanisms of nitrogen fixation.
摘要
- 来自褐球固氮菌的固氮制剂对以下底物进行还原，其米氏常数（K(m)）值如下：甲基异氰化物，1.8×10⁻⁴m；乙基异氰化物，2.5×10⁻²m；氰离子，1.4×10⁻³m；乙炔，1.2×10⁻⁴m。2. 氮气、一氧化碳或氢气竞争性抑制异氰化物还原，其抑制常数（K(i)）值如下：氢气，1.3×10⁻³m；一氧化碳，6.8×10⁻⁶m；氮气，4.3×10⁻⁴m。3. 活的固氮细菌以及分离出的三叶草根瘤可由甲基异氰化物形成甲烷。4. 结合其他研究工作及固氮的可能机制对这些结果进行了讨论。
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