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鸡蛋黄中的嘌呤N1-C6水解酶活性:一种残留酶?

A purine N1-C6 hydrolase activity in the chicken egg yolk: a vestigial enzyme?

作者信息

De Boeck S, Stockx J

出版信息

Enzyme. 1978;23(1):56-63. doi: 10.1159/000458550.

DOI:10.1159/000458550
PMID:564265
Abstract

All attempts to prove the presence of xanthine oxidase and uricase in yolk preparations failed. We were able instead to show that yolk preparations could hydrolyze the N1-C6 bond of certain purine bases. In the case of xanthine, 4-ureido-imidazole-5-carboxylic acid and 4-ureido-imidazole are formed. Activity only becomes apparent during purification. An analogous enzyme was described earlier in Clostridium cyclindrosporum. The liver and the blood plasma of actively laying hens do not contain the enzyme. A scheme for the degradation of egg ribonucleic acids is presented.

摘要

所有证明蛋黄制品中存在黄嘌呤氧化酶和尿酸酶的尝试均告失败。相反,我们能够证明蛋黄制品可以水解某些嘌呤碱的N1-C6键。就黄嘌呤而言,会形成4-脲基咪唑-5-羧酸和4-脲基咪唑。该活性仅在纯化过程中才会显现出来。之前在柱状芽孢梭菌中曾描述过一种类似的酶。正在产蛋的母鸡的肝脏和血浆中不含这种酶。本文还给出了鸡蛋核糖核酸的降解示意图。

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A purine N1-C6 hydrolase activity in the chicken egg yolk: a vestigial enzyme?鸡蛋黄中的嘌呤N1-C6水解酶活性:一种残留酶?
Enzyme. 1978;23(1):56-63. doi: 10.1159/000458550.
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[Scury and purine catabolism].[坏血病与嘌呤分解代谢]
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Digestion of purine ribonucleotides by intestinal enzymes of the developing rat fetus.发育中大鼠胎儿肠道酶对嘌呤核糖核苷酸的消化作用。
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Purine degradation in Pseudomonas aeruginosa and Pseudomonas testosteroni.铜绿假单胞菌和睾丸酮假单胞菌中的嘌呤降解
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RNA and purine metabolism during development of the normal and dystrophic chicken.正常和营养不良鸡发育过程中的RNA与嘌呤代谢
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Developmental changes of adenosine deaminase, xanthine oxidase, and uricase in mouse tissues.
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[The postnatal development of porcine purine catabolism and the effect of the environmental temperature on turnover].[猪嘌呤分解代谢的产后发育及环境温度对周转率的影响]
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Biochemical and genetical studies of purine breakdown in Aspergillus.曲霉属中嘌呤分解代谢的生化与遗传学研究。
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Amylase activity increases in the yolk of fertilized eggs during incubation in chickens.在鸡的孵化过程中,受精卵卵黄中的淀粉酶活性会增加。
Poult Sci. 1991 Oct;70(10):2176-9. doi: 10.3382/ps.0702176.

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