Vinklárková K, Sladký Z
Folia Microbiol (Praha). 1978;23(1):55-9. doi: 10.1007/BF02876596.
The mycelium of the fungus Pleurotus ostreatus was cultured on a synthetic medium with growth regulators. Best growth was observed on media with 100 ppm IAA, 200 ppm GA3 and 200 ppm of kinetin. Growth of terminal hyphae and the appearance of colonies differed characteristically. Auxin bio-assays showed that the content of substances in younger mycelium slowly increased during cultivation without conspicuous changes in the medium. After exogenous application of IAA the content of auxins in younger mycelium increased and the content of IAA decreased gradually in the culture medium. Gibberellin bio-assays showed their content in the mycelium fell during cultivation and while it increased in the medium. Application of GA3 to the medium leads to a significant increase in these substances in the young mycelium and in the medium their level remains the same. Cytokinin bio-assays showed their content to vary during cultivation. An addition to kinetin to the nutrient medium results in an increase of cytokinins in the mycelium while in the medium the level of cytokinins is maintained. Addition of one substance thus influences the levels of other endogenous regulators.
平菇菌丝体在添加了生长调节剂的合成培养基上培养。在含有100 ppm吲哚乙酸（IAA）、200 ppm赤霉素（GA3）和200 ppm激动素的培养基上观察到最佳生长情况。末端菌丝的生长和菌落的外观具有明显特征。生长素生物测定表明，在培养过程中，较年轻菌丝体中物质的含量缓慢增加，而培养基中没有明显变化。外源施加IAA后，较年轻菌丝体中生长素的含量增加，而培养基中IAA的含量逐渐降低。赤霉素生物测定表明，培养过程中菌丝体中赤霉素的含量下降，而培养基中其含量增加。向培养基中添加GA3会导致较年轻菌丝体中这些物质显著增加，且培养基中其水平保持不变。细胞分裂素生物测定表明，其含量在培养过程中有所变化。向营养培养基中添加激动素会导致菌丝体中细胞分裂素增加，而培养基中细胞分裂素的水平保持不变。因此，添加一种物质会影响其他内源调节剂的水平。
