Davies K E, Walker I O
J Cell Sci. 1978 Feb;29:93-102. doi: 10.1242/jcs.29.1.93.
A method is described for the isolation of pulse-labelled RNA from nuclei and subnuclear fractions of Physarum polycephalum. At all times during interphase the nucleolar RNA consisted mainly of a 34-S rRNA precursor with only small amounts of 26-S and 19-S rRNAs. The nucleoplasmic RNA consisted of predominantly mature 26-S rRNA with small amounts of 19-S rRNA and a 30-S RNA species. The 30-S RNA component displayed different labelling kinetics from the rRNA precursors. A low molecular weight RNA fraction (4-8 S) appeared to accumulate in the nucleus as interphase progressed.
本文描述了一种从多头绒泡菌的细胞核和亚核组分中分离脉冲标记RNA的方法。在间期的所有时间里，核仁RNA主要由34-S rRNA前体组成，只有少量的26-S和19-S rRNA。核质RNA主要由成熟的26-S rRNA组成，还有少量的19-S rRNA和一种30-S RNA组分。30-S RNA组分与rRNA前体表现出不同的标记动力学。随着间期的进行，一种低分子量RNA组分（4-8 S）似乎在细胞核中积累。
