多头绒泡菌变形体和原质团中转运RNA和核糖体RNA基因的组织方式。

The organisation of genes for transfer RNA and ribosomal RNA in amoebae and plasmodia of Physarum polycephalum.

Hall L, Braun R

Eur J Biochem. 1977 Jun 1;76(1):165-74. doi: 10.1111/j.1432-1033.1977.tb11582.x.

Abstract

Using hybridisation techniques nuclei from both amoebae and plasmodia of Physarum polycephalum were found to contain 275 genes each coding for 5.8-S, 19-S and 26-S rRNA, 685 genes for 5-S rRNA and 1050 genes for tRNA. 2. Hybridisation of these RNA species to both amoebal and plasmodial DNA fractionated on CsCl gradients reveal that the 5.8-S, 19-S and 26-S rRNA genes are located at a satellite position (formula: see text) with respect to the main band of DNA, whereas 4-S RNA genes are located exclusively in the main band of DNA (formula: see text). 3. This result was confirmed by demonstrating that only the 5.8-S, 19-S and 26-S rRNA species hybridise to purified plasmodial ribosomal DNA. 4. The 19-S and 26-S rRNA genes of amoebae are located on extrachromosomal DNA molecules of a discrete size (Mr = 38 X 10(6)) with identical properties to plasmodial ribosomal DNA.

摘要

运用杂交技术发现，多头绒泡菌的变形虫和原质团的细胞核均含有275个基因，每个基因编码5.8 - S、19 - S和26 - S核糖体RNA，685个基因编码5 - S核糖体RNA，1050个基因编码转运RNA。2. 这些RNA种类与在氯化铯梯度上分级分离的变形虫和原质团DNA杂交，结果显示，5.8 - S、19 - S和26 - S核糖体RNA基因相对于DNA主带位于卫星位置（公式：见正文），而4 - S RNA基因仅位于DNA主带（公式：见正文）。3. 通过证明只有5.8 - S、19 - S和26 - S核糖体RNA种类能与纯化的原质团核糖体DNA杂交，这一结果得到了证实。4. 变形虫的19 - S和26 - S核糖体RNA基因位于大小离散的染色体外DNA分子上（Mr = 38×10⁶），其性质与原质团核糖体DNA相同。