Lambadarios C, Hastings C, Abo-Darub J, Cooke I D
J Reprod Fertil. 1976 Mar;46(2):383-94. doi: 10.1530/jrf.0.0460383.
Human endometrium from the secretory phase of the menstrual cycle was incubated with 3H- and 14C-labelled glucosamine and [3H]leucine. Incorporation into secreted extracellular glycoprotein and accumulation of the label into the microsomal fraction were measured. When oestradiol or progesterone were added to the medium, medroxyprogesterone acetate (MPA), ethynodiol diacetate and chlormadinone acetate reduced incorporation of glucosamine and MPA reduced incorporation of leucine into glycoprotein. MPA reduced the amount of glucosamine in the microsomal fraction and also had an effect on amino acid transport within the endometrial cells, as indicated by intracellular alpha-aminoisobutyric acid space measurements. These results and the ratios of 3H and 14C in the microsomal fraction and secreted protein suggest that MPA has a primary effect in decreasing amino sugar incorporation and a secondary effect in reducing amino acid incorporation into glycoprotein.
将处于月经周期分泌期的人子宫内膜与3H和14C标记的葡糖胺以及[3H]亮氨酸一起孵育。测定了其掺入分泌的细胞外糖蛋白的情况以及标记物在微粒体部分的积累。当向培养基中添加雌二醇或孕酮时，醋酸甲羟孕酮（MPA）、醋酸炔诺二醇和醋酸氯地孕酮会降低葡糖胺的掺入，而MPA会降低亮氨酸掺入糖蛋白的量。MPA减少了微粒体部分中葡糖胺的量，并且如细胞内α-氨基异丁酸空间测量所示，对子宫内膜细胞内的氨基酸转运也有影响。这些结果以及微粒体部分和分泌蛋白中3H与14C的比率表明，MPA的主要作用是减少氨基糖的掺入，其次要作用是减少氨基酸掺入糖蛋白。
