Yamamoto R, Munakata H, Yamamoto M, Yosizawa Z
J Biochem. 1978 Feb;83(2):537-42. doi: 10.1093/oxfordjournals.jbchem.a131941.
A crude microsomal fraction (M-Fr) was separated from the endometrial scrapings of uteri of ovariectomized rabbits with or without hormonal treatment. The effects of estrogen and progesterone on the incorporation into M-Fr of L-[U-14C]-fucose and N-acetyl-D-[6-3H]-glucosamine from their nucleotides were investigated. Estrogen increased the incorporation of these sugars, whereas progesterone suppressed this effect. The results of fractionation on a DEAE-Sephadex A-25 (Cl- form) column of the isotope-labelled complex saccharide mixtures, obtained by pronase digestion of the incubation mixtures, indicated that biosynthesis of sulfated glycoprotein was most sensitive to the hormones among the complex saccharides in M-Fr. Thus, a hormonal effects on the biosynthesis of sulfated glycoprotein in the endometrium of ovariectomized rabbit has been unambiguously confirmed at the microsomal level.
从接受或未接受激素处理的去卵巢兔子宫的子宫内膜刮片中分离出粗微粒体部分（M-Fr）。研究了雌激素和孕酮对L-[U-¹⁴C]-岩藻糖和N-乙酰-D-[6-³H]-葡萄糖胺从其核苷酸掺入M-Fr的影响。雌激素增加了这些糖的掺入，而孕酮则抑制了这种作用。通过对孵育混合物进行链霉蛋白酶消化获得的同位素标记复合糖混合物在DEAE-葡聚糖A-25（Cl⁻形式）柱上的分级分离结果表明，在M-Fr中的复合糖中，硫酸化糖蛋白的生物合成对激素最为敏感。因此，已在微粒体水平明确证实激素对去卵巢兔子宫内膜中硫酸化糖蛋白生物合成的影响。
